Ddx4-creert2
WebDdx4-Cre小鼠与FloxP小鼠交配后，在Cre特异表达的组织或者器官或细胞中，两个FloxP位点间的外显子或元件将被删除，达到条件性敲除或过表达的目的。可用于生殖系统等相 … WebCre driver mice for germ cell lineage. In October 2024 "Mouse of the Month", we introduced a Ddx4-CreERT2 KI mouse that expresses tamoxifen-induced Cre recombinase under the control of endogenous Ddx4 promoter deposited by Seiya Mizuno, Ph.D.(University of Tsukuba). This strain is a useful tool for analyzing mechanisms of female oogenesis and …
Ddx4-creert2
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WebApr 15, 2024 · A male-sterile phenotype was similarly observed in the tamoxifen-inducible Ddx4 em1(CreERT2)Utr Cre allele, in which the transgene targeting design is highly … WebSKP1 localizes to synapsed chromosome axes in meiotic germ cells and specifically to the lateral elements. SKP1 is an essential gene and its global knockout leads to embryonic lethality (2). We investigated the role of SKP1 in male meiosis by an tamoxifen-inducible (Ddx4-CreERT2) deletion approach that we previously used effectively.
WebWe mated the CreERT2 mice with R26GRR mice, expressing enhanced green fluorescent protein (EGFP) and tDsRed before and after Cre recombination. We found tDsRed signals in the testes and ovaries of tamoxifen‐treated B6‐Ddx4 em1(CreERT2)Utr::R26GRR mice, but not in untreated mice. Immunostaining of their ovaries clearly showed that Cre ... Web什么是CreERT2 ？. 将人雌激素受体（estrogen receptor，简称ER）的配体结合区（LBD）与Cre 重组酶相融合，形成定位于胞浆中的融合蛋白（Cre-ER）。. 只有在雌激 …
WebApr 6, 2009 · The CreERT2 protein was ubiquitously expressed from the Rosa26 locus but repressed by the Hsp90 complex. Tamoxifen binding releases CreERT2 from Hsp90 to permit Cre recombination of the loxP sites surrounding exon 2 of Mll2, ... Ddx4 and Dazl were both high on the list of down-regulated genes from the ES cell expression profiling. WebDdx4 was specifically expressed in both female and male germ cell lineages. We mated the CreERT2 mice with R26GRR mice, expressing enhanced green fluorescent protein …
WebYthdc2fl/- and Ythdc2 fl/- Ddx4-CreERT2 males between 10-25 weeks were injected with tamoxifen (2 mg/30 g body weight) daily for five consecutive days. At 2 days post treatment (2 dpt), pachytene spermatocytes were purified by Staput. Growth protocol: Pachytene spermatocytes were purified by Staput (sedimentationt at unit gravity) Extracted ...
http://www.larc.tsinghua.edu.cn/post/971/31 壺 2ちゃんねるWebFree math problem solver answers your algebra, geometry, trigonometry, calculus, and statistics homework questions with step-by-step explanations, just like a math tutor. box drive インストール windows11WebOct 1, 2011 · Introduction. DDX4 (DEAD [Asp-Glu-Ala-Asp] box polypeptide 4), also known as VASA, is a highly conserved component of the germ plasm. Vasa was originally identified in Drosophila as a maternal effect gene required for the formation of abdominal segments and germ cell specification [].Since then, it has been identified in the germ cells of a large … box drive インストール windowshttp://www.larc.tsinghua.edu.cn/post/971/31 売買契約書 テンプレートWebThe Ddx4 -CreERT2 KI mouse (RBRC11094) is a Cre driver mouse that expresses tamoxifen-induced Cre recombinase under the control of endogenous Ddx4 promoter. … 売茶翁 みちのくせんべい 価格WebOur functional assessment of this new allele, designated Ddx4 iCreJoBo, reveals that Cre activity begins in PGCs from at least E10.5, and that it achieves higher efficiency for early gonadal (E10.5-12.5) germline deletion when compared to the inducible Oct4 CreERT2 line. We found the Ddx4 iCreJoBo allele to be hypomorphic for Ddx4 expression ... 売電単価とはWebDec 14, 2024 · To circumvent the early meiotic block in both global and conditional knockout Ythdc2 mutants, we generated Ythdc2 fl/-Ddx4-Cre ERT2 mice (referred to as Ythdc2 iKO) for tamoxifen-inducible deletion in germ cells (Figure 1 A). Ddx4-Cre ERT2 is expressed in both mitotic and meiotic germ cells (John et al., 2008).Intraperitoneal tamoxifen injection … 壹 読み