Fos-creert2
WebI want to use TRAP2 mice (Fos-CreERT2) and viral injections of AAV-DIO-fluorescent protein to tag and detect memory-related ensemble neurons. In all papers I found with similar approaches, the... WebPlasmid pCAG-ERT2CreERT2 from Dr. Connie Cepko's lab contains the insert ERT2-Cre-ERT2 fusion protein and is published in Proc Natl Acad Sci U S A. 2007 Jan 5. ():. This plasmid is available through Addgene.
Fos-creert2
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WebJan 18, 2024 · To test the causal role of the BLA nociceptive ensemble for pain behaviors, we expressed a Cre-dependent inhibitory DREADD neuromodulator (hM4-mCherry) in mutant TRAP mice ( FosCreERT2) by applying noxious pin pricks that induced activity-dependent, spatially, and temporally controlled DNA recombination and hM4-mCherry … WebNational Center for Biotechnology Information
WebFos-CreERT2 Models U-GEMMs Toolbox of Mice Find Cre Reporters CRISPR/Cas knockins Tet-regulated Disease Mice Spontaneous Tumor Rare Disease Infectious … WebFostm1.1(cre/ERT2)LuoTargeted Allele Detail Summary Mutation origin Mutation description Recombinase activity Phenotypes Find Mice (IMSR) References Summary …
WebMay 18, 2012 · CreERT2 has high affinity for the 4-hydroxytamoxifen (4-OHT) but not for the endogenous estradiol. After its exposure to the specific inducer, CreERT2 is translocated into the nucleus and mediates the recombination of floxed genes. WebJun 11, 2024 · When these mice are fasted to enter torpor, the neurons that are active induce FOS and CreERT2. When these mice are exposed to …
WebFos-creERT2,eWAT, hM3D-3 Sample type SRA Source name visceral epididymal WAT (eWAT) Organism Mus musculus Characteristics tissue: visceral epididymal WAT (eWAT) strain: Fos-creERT2 stress: repeated activaiton of PVN neurons time: every 3 days for 42 days Extracted molecule total RNA Extraction protocol
WebFor this experiment, we used mice harbouring a tamoxifen-dependent form of Cre recombinase driven from the Fos locus (Fos 2A-iCreERT2, TRAP2 36) together with an allele of the Gq-coupled... brown rice protein oxalateWebFos-CreERT2, Fos em1(CreERT2-SV40-pA)Smoc: Gene: Fos Location: Chr12:85520664-85524047 bp, + strand Genetic Position: Chr12, 39.7 cM Alliance: Fos em1(cre/ERT2)Smoc page Mutation origin: Strain of Origin: C57BL/6J: Mutation description: Allele Type: Endonuclease-mediated (Inducible, Recombinase) every person is like some other personWebWe used Fos CreERT2/+ /Ai14 transgenic mice to tag cells activated by and potentially encoding cocaine and sucrose seeking. We tagged ~1% of neurons in the core subregion of the accumbens (NAcore) activated during cue-induced seeking for cocaine or sucrose. The majority of tagged cells in the seeking ensembles were D1-MSNs, and specifically ... brown rice protein powder factoryWebFeb 17, 2024 · Fos. CreERT2 is expressed under c-Fos promotor but is retained in the cytoplasm when 4-OHT is absent. Upon injection of 4-OHT, CreERT2 enters the nucleus, excises the STOP-cassette and enables tdTomato expression. (b) Exemplary expression patterns of control (left; n = 3) and rotarod trained mice (right; n = 3). every person plans to run offWebOct 29, 2024 · We used Fos CreERT2/+ /Ai14 transgenic mice to tag cells activated by and potentially encoding cocaine and sucrose seeking. We tagged ~1% of neurons in the core subregion of the accumbens (NAcore) activated during cue-induced seeking for cocaine or sucrose. The majority of tagged cells in the seeking ensembles were D1-MSNs, and … brown rice protein bar recipeWebDec 4, 2024 · Here, we performed Fos-CreERT2–based activity mapping, correlated for pregnant mouse dams and their fetuses with amphetamine, nicotine, and caffeine applied acutely during midgestation. While light-sheet microscopy-assisted intact tissue imaging revealed drug- and age-specific neuronal activation, the indusium griseum (IG) appeared ... every person s life is worth a novelWebView 7 photos for 482 Fall Creek Cir, Goodlettsville, TN 37072, a 3 beds, 2 baths, 2177 Sq. Ft. rental home with a rental price of $2400 per month. Browse property photos, details, … brown rice protein powder australia